A8599
Sigma
Monoclonal Anti-Apolipoprotein E antibody produced in mouse
~1 mg/mL, clone E6D7, purified immunoglobulin, buffered aqueous solution
| Synonym: | Anti-Apo E |
| MDL number: | MFCD01864094 |
Description
| Immunogen | synthetic peptide spanning the polymorphic amino acid position 158 of ApoE. |
| Specificity | Shown to enhance the risk for the development of Alzheimers's disease. Mutations in the APP gene in conjunction with the influence of ApoE appears to influence amyloid metabolism. Reacts to the E2, E3, and E4 isoforms of apoliporotein E. |
| Physical form | Solution in phosphate buffered saline. |
Properties
| antibody form | purified immunoglobulin |
| clone | E6D7 (monoclonal) |
| form | buffered aqueous solution |
| species reactivity | human |
| concentration | ~1 mg/mL |
| application(s) | immunoblotting: 1:102-1:103 |
| immunohistochemistry: 1:100-1:10,000 using fixed tissues pretreated with 70% formic acid for 10-30 minutes at room temperature. | |
| immunoprecipitation: suitable | |
| indirect ELISA: suitable | |
| isotype | IgG1 |
| Gene Information | human ... APOE(348) |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 2 |
References
| reference | Norman, R.R., et al., Apolipoprotein E geneotyping in Alzheimer's disease. Ann. N.Y. Acad. Sci. 802 |
Akima, M., et al., Ubiquitin-positive inclusions in ependymal cells. Acta Neuropathol. 106, 129-136, (2003) Abstract |
